Opp M R, Krueger J M
Department of Physiology and Biophysics, University of Tennessee, Memphis 38163.
Brain Res. 1994 Mar 7;639(1):57-65. doi: 10.1016/0006-8993(94)91764-7.
Interleukin-1 (IL-1) is hypothesized to be involved in sleep regulation. Antibodies directed against interleukin-1 beta (anti-IL-1 beta) were injected intracerebroventricularly (ICV) into normal rabbits to determine the effects of the reduction of endogenous IL-1 beta on spontaneous sleep/wake behavior. A 100 micrograms dose of anti-IL-1 beta reduced non-rapid eye movements sleep (NREMS) by about 20-min during the first 4-h postinjection without affecting REMS, amplitudes of electroencephalographic (EEG) slow waves, or brain temperature. Plasma concentrations of IL-1 beta, as determined by radioimmunoassay, were detectable in 39 of 79 (49%) blood samples taken before and after 4-h total sleep deprivation (SD). Plasma concentrations of IL-1 beta were, on average, significantly elevated following SD. There was a rebound in NREMS duration and of amplitudes of EEG slow waves of about 2-h duration following SD. This rebound in both parameters was exaggerated if rabbits received vehicle or anti-IL-1 beta in conjunction with SD. However, ICV administration of anti-IL-1 beta at the beginning or end of the SD period attenuated, relative to values obtained after administration of vehicle, the NREMS rebound following SD. It is concluded that SD alters the responsiveness of rabbits to experimental manipulation and that responses to SD in the rabbit are mediated, in part, by IL-1.
白细胞介素-1（IL-1）被认为参与睡眠调节。将抗白细胞介素-1β（抗IL-1β）抗体脑室内注射（ICV）到正常兔子体内，以确定内源性IL-1β减少对自发睡眠/觉醒行为的影响。注射100微克抗IL-1β后，在最初4小时内非快速眼动睡眠（NREMS）减少约20分钟，而不影响快速眼动睡眠（REMS）、脑电图（EEG）慢波振幅或脑温。通过放射免疫测定法测定，在4小时总睡眠剥夺（SD）前后采集的79份血样中，有39份（49%）可检测到血浆IL-1β浓度。总睡眠剥夺后，血浆IL-1β浓度平均显著升高。睡眠剥夺后，NREMS持续时间和EEG慢波振幅有大约2小时的反弹。如果兔子在睡眠剥夺时同时接受赋形剂或抗IL-1β，这两个参数的反弹会加剧。然而，相对于给予赋形剂后获得的值，在睡眠剥夺期开始或结束时脑室内给予抗IL-1β会减弱睡眠剥夺后的NREMS反弹。得出的结论是，睡眠剥夺会改变兔子对实验操作的反应性，并且兔子对睡眠剥夺的反应部分由IL-1介导。
