Natvig J B, Kunkel H G, Rosenfield R E, Dalton J F, Kochwa S
J Immunol. 1976 Jun;116(6):1536-8.
Idiotypic antibodies were produced in rabbits to Rh antibodies isolated from the serum of an individual with high Rh antibody titer. These antisera after absorption with blood IgG failed to react with immunoglobulins lacking anti-Rh activity. They agglutinated cells coated with the immunizing antibody to high titers. Two other anti-Rh coats out of 22 tested also were agglutinated but to considerably reduced titers indicating a degree of anti-Rh cross specificity. Four other antisera made to red cells coated with different anti-Rh antibodies showed definite but weaker idiotypic specificity. Both the idiotypic and cross-idiotypic antigens of the Rh antibodies were completely blocked when univalent fragments of the Rh antibodies were bound to antigen on the red cell.
用兔制备独特型抗体，所用的Rh抗体是从一名Rh抗体效价高的个体血清中分离出来的。这些抗血清用血液IgG吸收后，不再与缺乏抗Rh活性的免疫球蛋白发生反应。它们能使包被有免疫抗体的细胞发生高滴度凝集。在22种测试的其他抗Rh包被中，有两种也发生了凝集，但滴度显著降低，表明存在一定程度的抗Rh交叉特异性。另外四种针对包被有不同抗Rh抗体的红细胞制备的抗血清显示出明确但较弱的独特型特异性。当Rh抗体的单价片段与红细胞上的抗原结合时，Rh抗体的独特型和交叉独特型抗原都被完全阻断。
