Schneemann A, Schneider P A, Kim S, Lipkin W I
Department of Neurology, University of California, Irvine 92717.
J Virol. 1994 Oct;68(10):6514-22. doi: 10.1128/JVI.68.10.6514-6522.1994.
Borna disease virus (BDV) is a nonsegmented, negative-strand RNA virus that causes neurologic disorders in a wide range of animal species. Although the virus is unclassified, sequence analysis of the 8.9-kb viral genome has shown that it is related to rhabdoviruses and paramyxoviruses. We have mapped subgenomic RNAs of BDV strain He80-1 to the viral genome by determining the precise sequences at their 5' and 3' termini. This analysis showed that the genome contains three transcription initiation sites and four termination sites. A 14- to 16-nucleotide semiconserved sequence was present at the gene start sites and partially copied into the subgenomic RNAs. The termination sites contained a U-rich motif reminiscent of termination signals in rhabdoviruses and paramyxoviruses. In contrast to the genomes of other nonsegmented, negative-strand RNA viruses, the BDV genome lacked the typical configuration of termination signal, intergenic region, and initiation signal at the gene boundaries. Instead, transcription units and transcription signals frequently overlapped. These differences have implications for our understanding of the control of viral transcription and may relate to the low-level replication and persistence of BDV.
博尔纳病病毒（BDV）是一种不分节段的负链RNA病毒，可在多种动物物种中引发神经紊乱。尽管该病毒未被分类，但对其8.9 kb病毒基因组的序列分析表明，它与弹状病毒和副粘病毒有关。我们通过确定BDV毒株He80 - 1的亚基因组RNA 5'和3'末端的精确序列，将其定位到病毒基因组上。该分析表明，基因组包含三个转录起始位点和四个终止位点。在基因起始位点存在一个14至16个核苷酸的半保守序列，并部分复制到亚基因组RNA中。终止位点含有一个富含U的基序，让人联想到弹状病毒和副粘病毒中的终止信号。与其他不分节段的负链RNA病毒基因组不同，BDV基因组在基因边界处缺乏终止信号、基因间隔区和起始信号的典型结构。相反，转录单元和转录信号经常重叠。这些差异对于我们理解病毒转录的控制具有重要意义，并且可能与BDV的低水平复制和持续性有关。
