Nagano K, Nakura J, Kihara K, Ye L, Kamino K, Mitsuda N, Ohta T, Jinno Y, Niikawa N, Miki T
Department of Geriatric Medicine, Osaka University Medical School, Japan.
Jpn J Hum Genet. 1993 Dec;38(4):391-7. doi: 10.1007/BF01907985.
We have constructed a new genetic linkage map of the Werner syndrome (WRN) region, using microsatellites from a library which was developed by a chromosome microdissection and enzymatic amplification method. These microsatellites were used to genotype members of CEPH families using a simplified detection system of polymerase chain reaction (PCR) products. Two-point analysis was used to assign 4 microsatellite markers relative to each marker and other markers reported in the CEPH public data base. We confirmed that these 4 markers are located to the WRN region, 8p11.2-p22. Such microsatellites microdissected from the definite chromosome region may be useful for positional cloning.
我们构建了一个新的沃纳综合征（WRN）区域的遗传连锁图谱，使用的微卫星来自一个通过染色体显微切割和酶促扩增方法构建的文库。这些微卫星用于通过聚合酶链反应（PCR）产物的简化检测系统对CEPH家系成员进行基因分型。采用两点分析来确定相对于每个标记以及CEPH公共数据库中报告的其他标记的4个微卫星标记。我们证实这4个标记位于WRN区域，即8p11.2 - p22。从特定染色体区域显微切割得到的此类微卫星可能对定位克隆有用。
