老年BALB/c小鼠的仙台病毒性肺炎

Sendai viral pneumonia in aged BALB/c mice.

作者信息

Jacoby R O, Bhatt P N, Barthold S W, Brownstein D G

机构信息

Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut 06510.

出版信息

Exp Gerontol. 1994 Jan-Feb;29(1):89-100. doi: 10.1016/0531-5565(94)90065-5.

DOI:10.1016/0531-5565(94)90065-5

PMID:8187844

Abstract

Sendai virus (SV) infection in aged BALB/c mice was evaluated as a natural model for age-associated susceptibility to viral pneumonia. Young (2 month-old) and aged (22-24 month-old) BALB/c mice were inoculated intranasally with 100 median pneumonia doses (PD50) of SV and examined at 6, 10, and 20 days by virus titration, immunohistochemistry, histopathology, and serology. The aged mice had significantly higher virus titers in lung, prolonged infection, delayed development, and resolution of pneumonia and significantly lower serum antibody titers. In a second experiment, the responses of young mice were compared to intermediate-aged mice (11-13 and 17-18 months old). The intermediate-aged mice had some characteristics of young mice and others of aged mice. The results indicate that SV infection can be used to study aging-associated susceptibility to a pneumotropic virus in a natural host, and that susceptibility of mice to viral pneumonia increases gradually during aging.

摘要

将仙台病毒（SV）感染老年BALB/c小鼠作为与年龄相关的病毒性肺炎易感性的天然模型进行评估。将年轻（2月龄）和老年（22 - 24月龄）BALB/c小鼠经鼻接种100个中位数肺炎剂量（PD50）的SV，并在第6、10和20天通过病毒滴定、免疫组织化学、组织病理学和血清学进行检查。老年小鼠肺部病毒滴度显著更高，感染持续时间延长，肺炎发展和消退延迟，血清抗体滴度显著更低。在第二个实验中，将年轻小鼠的反应与中年小鼠（11 - 13月龄和17 - 18月龄）进行比较。中年小鼠具有一些年轻小鼠的特征和一些老年小鼠的特征。结果表明，SV感染可用于研究天然宿主中与衰老相关的对嗜肺病毒的易感性，并且小鼠对病毒性肺炎的易感性在衰老过程中逐渐增加。