Nicklin M J, Weith A, Duff G W
Department of Medicine and Pharmacology, University of Sheffield, Royal Hallamshire Hospital, United Kingdom.
Genomics. 1994 Jan 15;19(2):382-4. doi: 10.1006/geno.1994.1076.
We have constructed a restriction map of the human genomic region containing the genes encoding the three members of the interleukin-1 (IL-1) family, IL-1 alpha, IL-1 beta, and IL-1 receptor antagonist (IL-1 ra). For this purpose, pulsed-field gel electrophoresis blots were hybridized with probes derived from the three genes. The genes (IL1A, IL1B, and IL1RN, respectively) were found to map to a common restriction fragment of approximately 430 kb that is flanked by two clusters of sites for methylation-sensitive rare-cutter restriction enzymes (putative CpG islands). A likely third internal CpG island was marked by two rare-cutter sites. CpG and non-CpG-specific enzymes were used to map the three genes. Relative to one terminal CpG island, the three genes were mapped to the following intervals: IL1A was between +0 and +35 kb, IL1B was between +70 and +110 kb, and IL1RN was between +330 and +430 kb.
我们构建了人类基因组区域的限制酶切图谱，该区域包含编码白细胞介素-1（IL-1）家族三个成员的基因，即IL-1α、IL-1β和IL-1受体拮抗剂（IL-1ra）。为此，用来自这三个基因的探针与脉冲场凝胶电泳印迹进行杂交。发现这些基因（分别为IL1A、IL1B和IL1RN）定位于一个约430kb的共同限制片段上，该片段两侧是两簇对甲基化敏感的稀有切割限制酶位点（推定的CpG岛）。一个可能的第三个内部CpG岛由两个稀有切割位点标记。使用CpG特异性和非CpG特异性酶对这三个基因进行定位。相对于一个末端CpG岛，这三个基因定位于以下区间：IL1A在+0至+35kb之间，IL1B在+70至+110kb之间，IL1RN在+330至+430kb之间。
