Mellado R P, Vinuela E, Salas M
Eur J Biochem. 1976 May 17;65(1):213-23. doi: 10.1111/j.1432-1033.1976.tb10408.x.
By treatment of Bacillus subtilis MO-101-P spoA- met thr- su- with ethyl methanesulfonate, a strong suppressor strain of nonsense mutations, B. subtilis MO-101-P spoA- [met-]+thr- su+44, was isolated. This strain does not suppress phage phi 29 mutant susB47, selected on a B. subtilis strain containing the su+3 suppressor isolated by Georgopoulos. A revertant from this mutant, susB610, was isolated, being suppressed by both the su+3 and su+44 suppressor strains. The efficiency of suppression by strain su+44 is about 50%. The experiments shown in this paper suggest that strain su+44 contains an amber and strain su+3 an ochre suppressor.
用甲磺酸乙酯处理枯草芽孢杆菌MO-101-P spoA- met thr- su-，分离得到一种无义突变的强抑制菌株，即枯草芽孢杆菌MO-101-P spoA- [met-]+thr- su+44。该菌株不能抑制在含有由乔治opoulos分离得到的su+3抑制子的枯草芽孢杆菌菌株上选择的噬菌体φ29突变体susB47。从该突变体中分离出一个回复突变体susB610，它能被su+3和su+44抑制菌株所抑制。su+44菌株的抑制效率约为50%。本文所示的实验表明，su+44菌株含有一个琥珀型抑制子，而su+3菌株含有一个赭石型抑制子。
