Scharmann W, Jacob F, Porstendörfer J
J Gen Microbiol. 1976 Apr;93(2):303-8. doi: 10.1099/00221287-93-2-303.
Human polymorphonuclear leucocytes treated in vitro with leucocidin from Pseudomonas aeruginosa underwent characteristic morphological alterations as shown by phase-contrast and scanning electron microscopy. Within a few minutes of exposure to leucocidin the granulocytes became round, and protoplasmic extrusions appeared on the cell membrane, were withdrawn again and put out at another point of the cell. The final stage of the leucocidin-treated leucocyte was an enlarged, rounded vesicle with apparently intact plasma membrane. Omission of calcium ions from the diluting buffer caused certain differences in the morphologic appearance of the damaged leucocytes.
用铜绿假单胞菌白细胞毒素在体外处理的人多形核白细胞，经相差显微镜和扫描电子显微镜观察显示出特征性的形态学改变。在接触白细胞毒素几分钟内，粒细胞就变成圆形，细胞膜上出现原生质突出物，这些突出物又缩回，然后在细胞的另一点伸出。经白细胞毒素处理的白细胞的最后阶段是一个扩大的圆形囊泡，其质膜显然完整。稀释缓冲液中省去钙离子会导致受损白细胞的形态外观出现某些差异。
