Chaudhuri S, Messing J
Waksman Institute, Rutgers, State University of New Jersey, Piscataway, NJ 08855-0759.
Proc Natl Acad Sci U S A. 1994 May 24;91(11):4867-71. doi: 10.1073/pnas.91.11.4867.
Parental imprinting describes the phenomenon of unequivalent gene function based on transmission from the female or male parent. We have discovered parental imprinting of an allele of the dzr1 locus that posttranscriptionally regulates the accumulation of 10-kDa zein in the maize endosperm. The imprinted allele of MO17 inbred origin, dzr1 + MO17, conditions low accumulation of the 10-kDa zein and is dominant when transmitted through the female but recessive when transmitted through the male. Analyzing endosperms with equal parental contributions of dzr1 + MO17 ruled out the possibility that the unequivalent phenotype of dzr1 + MO17 was due to parental dosage imbalance in the triploid endosperm. Second-generation studies show that the dominant or recessive phenotype of dzr1 + MO17 is determined at every generation based on immediate parental origin with no grandparental effect.
亲本印记描述了基于来自雌性或雄性亲本的遗传而导致基因功能不等同的现象。我们发现了dzr1基因座一个等位基因的亲本印记，该基因座在转录后调控玉米胚乳中10 kDa醇溶蛋白的积累。自交系MO17来源的印记等位基因dzr1 + MO17，导致10 kDa醇溶蛋白的低积累，通过雌性传递时呈显性，而通过雄性传递时呈隐性。对具有相等亲本贡献的dzr1 + MO17胚乳进行分析，排除了dzr1 + MO17不等同表型是由于三倍体胚乳中亲本剂量不平衡所致的可能性。第二代研究表明，dzr1 + MO17的显性或隐性表型在每一代中都由直接亲本来源决定，不存在祖父母效应。
