Galambos R, Juhász G, Kékesi A K, Nyitrai G, Szilágyi N
Department of Neurosciences, University of California, San Diego, La Jolla 92093.
Proc Natl Acad Sci U S A. 1994 May 24;91(11):5153-7. doi: 10.1073/pnas.91.11.5153.
We show here electroretinograms (ERGs) recorded from freely moving rats during sleep and wakefulness. Bilateral ERGs were evoked by flashes delivered through a light-emitting diode implanted under the skin above one eye and recorded through electrodes inside each orbit near the optic nerve. Additional electrodes over each visual cortex monitored the brain waves and collected flash-evoked cortical potentials to compare with the ERGs. Connections to the stimulating and recording instruments through a plug on the head made data collection possible at any time without physically disturbing the animal. The three major findings are (i) the ERG amplitude during slow-wave sleep can be 2 or more times that of the waking response; (ii) the ERG patterns in slow-wave and REM sleep are different; and (iii) the sleep-related ERG changes closely mimic those taking place at the same time in the responses evoked from the visual cortex. We conclude that the mechanisms that alter the visual cortical-evoked responses during sleep operate also and similarly at the retinal level.
我们在此展示了在自由活动的大鼠睡眠和清醒期间记录的视网膜电图（ERG）。通过植入一只眼睛上方皮肤下的发光二极管发出的闪光诱发双侧ERG，并通过靠近视神经的每个眼眶内的电极进行记录。每个视觉皮层上方的附加电极监测脑电波并收集闪光诱发的皮层电位，以与ERG进行比较。通过头部的插头与刺激和记录仪器相连，使得在不实际干扰动物的情况下随时进行数据收集成为可能。三个主要发现是：（i）慢波睡眠期间的ERG振幅可以是清醒反应的2倍或更多；（ii）慢波睡眠和快速眼动睡眠中的ERG模式不同；（iii）与睡眠相关的ERG变化紧密模仿在同一时间从视觉皮层诱发的反应中发生的变化。我们得出结论，在睡眠期间改变视觉皮层诱发反应的机制在视网膜水平也同样起作用。
