荧光素标记青霉素的合成、纯化及动力学性质
Synthesis, purification and kinetic properties of fluorescein-labelled penicillins.
作者信息
Lakaye B, Damblon C, Jamin M, Galleni M, Lepage S, Joris B, Marchand-Brynaert J, Frydrych C, Frere J M
机构信息
Laboratoire d'Enzymologie and Centre d'ingénierie des protéines, Université de Liège, Sart-Tilman, Belgium.
出版信息
Biochem J. 1994 May 15;300 ( Pt 1)(Pt 1):141-5. doi: 10.1042/bj3000141.
Abstract
The synthesis and properties of six fluorescein-labelled penicillins are reported. The two isomers of fluoresceyl-glycyl-6-amino-penicillanic acid are probably the best compounds to use for detection of all the penicillin-binding proteins (PBPs) present in a bacterial membrane preparation. However, the derivatives of ampicillin were much more efficient against Enterobacter aerogenes PBP3. The two isomers obtained when a commercial mixture of the two isomers of carboxyfluorescein was used most often exhibited similar properties, but the Streptomyces R61 extracellular DD-peptidase was only efficiently acylated by the 5'-carboxyfluorescein derivative of glycyl-6-aminopenicillanic acid.
摘要
报道了六种荧光素标记青霉素的合成及性质。荧光素基甘氨酰-6-氨基青霉烷酸的两种异构体可能是用于检测细菌膜制剂中所有青霉素结合蛋白（PBPs）的最佳化合物。然而，氨苄西林的衍生物对产气肠杆菌PBP3的作用效率更高。当使用羧基荧光素两种异构体的商业混合物时得到的两种异构体通常表现出相似的性质，但链霉菌R61细胞外DD-肽酶仅被甘氨酰-6-氨基青霉烷酸的5'-羧基荧光素衍生物有效酰化。
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