Burczak K, Fujisato T, Hatada M, Ikada Y
Research Center for Biomedical Engineering, Kyoto University, Japan.
Biomaterials. 1994 Feb;15(3):231-8. doi: 10.1016/0142-9612(94)90072-8.
Hydrogel membranes were prepared by radiation and chemical cross-linking of poly(vinyl alcohol) (PVA) in aqueous solutions. Effects of PVA concentration, PVA molecular weight and radiation dose, as well as concentration of cross-linking agent, in the case of the chemical cross-linking procedure, on the permeation of insulin, albumin and immunoglobulin (IgG) through the membranes were investigated. Glucose permeation was also studied. The cross-linking density affected the size of the macromolecular mesh of the hydrogel network and thus the water content of the membrane responsible for the diffusion of the solutes. The diffusion coefficient linearly increased for all the solutes with increasing water content in the PVA hydrogels, indicating that diffusion occurs primarily through the water hydrating the polymer network. The permeability study showed that the water content as well as the mesh size had an influence on the diffusion of low molecular weight glucose and insulin. Although the diffusion of higher molecular weight solutes, such as albumin and IgG, was not so much affected by the mesh size of elaborated PVA hydrogel membranes, the diffusion of these proteins was very low.
水凝胶膜是通过在水溶液中对聚乙烯醇（PVA）进行辐射交联和化学交联制备的。研究了PVA浓度、PVA分子量、辐射剂量以及化学交联过程中交联剂浓度对胰岛素、白蛋白和免疫球蛋白（IgG）透过膜的渗透作用。还研究了葡萄糖的渗透情况。交联密度影响水凝胶网络大分子网格的大小，进而影响负责溶质扩散的膜的含水量。对于PVA水凝胶中所有溶质，随着含水量增加，扩散系数呈线性增加，表明扩散主要通过使聚合物网络水合的水发生。渗透性研究表明，含水量和网格大小对低分子量葡萄糖和胰岛素的扩散有影响。虽然较高分子量溶质（如白蛋白和IgG）的扩散受精心制备的PVA水凝胶膜网格大小的影响较小，但这些蛋白质的扩散非常低。
