Yoshikawa M, Yamaguchi S, Chatani N, Nishino Y, Matsuoka T, Yamahara J, Murakami N, Matsuda H, Kubo M
Kyoto Pharmaceutical University, Japan.
Yakugaku Zasshi. 1994 Apr;114(4):241-7. doi: 10.1248/yakushi1947.114.4_241.
As a series of study on the evaluation of Alismatis Rhizoma and the chemical characterization of the processing, a quantitative method by high performance liquid chromatography (HPLC) for ten triterpene constituents, alisols A, A monoacetate, B, B monoacetate, E 23-acetate, F, and G and 13,17-epoxyalisol A, 11-deoxyalisols B and B 23-acetate, has been developed. By the use of this HPLC method, the contents of these triterpenes in various Alismatis Rhizoma and the fresh rhizoma of Alisma oriental JUZEPC, originated from in China, Taiwan, and Japan were examined. Furthermore, the chemical change of the triterpene constituents during the drying process of the rhizoma of Alisma oriental has been investigated and it was found that the bioactive triterpenes of Alismatis Rhizoma such as alisol A and alisol A monoacetate were artificially formed during the drying process.
作为一系列关于泽泻评价及其炮制化学特征的研究，已开发出一种高效液相色谱（HPLC）定量方法，用于测定10种三萜成分，即泽泻醇A、泽泻醇A单乙酸酯、泽泻醇B、泽泻醇B单乙酸酯、泽泻醇E 23 - 乙酸酯、泽泻醇F、泽泻醇G以及泽泻环氧醇A、11 - 脱氧泽泻醇B和泽泻醇B 23 - 乙酸酯。利用该HPLC方法，检测了来自中国、台湾和日本的不同泽泻及东方泽泻新鲜根茎中这些三萜类化合物的含量。此外，还研究了东方泽泻根茎干燥过程中三萜成分的化学变化，发现泽泻的生物活性三萜类化合物如泽泻醇A和泽泻醇A单乙酸酯是在干燥过程中人工形成的。
