通过用浓碘化钠和十二烷基硫酸钠进行异丙醇分级分离从人全血中纯化基因组DNA。 - Suppr

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超能文献

Purification of genomic DNA from human whole blood by isopropanol-fractionation with concentrated Nal and SDS.

作者信息

Wang L, Hirayasu K, Ishizawa M, Kobayashi Y

机构信息

Osaka Research Laboratories, Wako Pure Chemical Industries, Ltd., Hyogo, Japan.

出版信息

Nucleic Acids Res. 1994 May 11;22(9):1774-5. doi: 10.1093/nar/22.9.1774.

DOI:10.1093/nar/22.9.1774

PMID:8202389

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC308067/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e6b/308067/b67d492eba57/nar00033-0273-a.jpg

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本文引用的文献

A simple salting out procedure for extracting DNA from human nucleated cells.一种从人有核细胞中提取DNA的简单盐析方法。

Nucleic Acids Res. 1988 Feb 11;16(3):1215. doi: 10.1093/nar/16.3.1215.

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.使用热稳定DNA聚合酶进行引物引导的DNA酶促扩增。

Science. 1988 Jan 29;239(4839):487-91. doi: 10.1126/science.2448875.

Simple procedure of DNA isolation from human serum.从人血清中分离DNA的简单方法。

Nucleic Acids Res. 1991 Oct 25;19(20):5792. doi: 10.1093/nar/19.20.5792.

Purification of human leucocyte DNA: proteinase K is not necessary.

Anal Biochem. 1992 Mar;201(2):362-5. doi: 10.1016/0003-2697(92)90352-8.

Purification of cloned and genomic DNA by guanidine thiocyanate/isobutyl alcohol fractionation.

Anal Biochem. 1992 Nov 15;207(1):197-201. doi: 10.1016/0003-2697(92)90523-a.

A general method for isolation of high molecular weight DNA from eukaryotes.一种从真核生物中分离高分子量DNA的通用方法。

Nucleic Acids Res. 1976 Sep;3(9):2303-8. doi: 10.1093/nar/3.9.2303.

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