Giraudo S Q, Kotz C M, Grace M K, Levine A S, Billington C J
Neuroendocrine Research Laboratory, Department of Veterans Affairs Medical Center, Minneapolis, Minnesota 55417.
Am J Physiol. 1994 May;266(5 Pt 2):R1578-83. doi: 10.1152/ajpregu.1994.266.5.R1578.
We measured the influence of diet composition on hypothalamic neuropeptide Y (NPY) message and brown fat uncoupling protein (UCP) mRNA using different diets. Sprague-Dawley rats ate ad libitum either chow, a high-carbohydrate (HC), an intermediate-carbohydrate (IHC), a high-fat (HF), or an intermediate-fat (IHF) diet, all with equal protein content (g/kcal). The HF and IHF groups ate less food mass and, except for HC, all groups consumed similar kilocalories during the study. After 1 wk, we killed the animals and extracted total RNA from arcuate nucleus, cortex, and brown adipose tissue (BAT). Arcuate NPY mRNA in the HF group was significantly (P < 0.001) lower than in the HC and chow group. There were no differences between groups in NPY message in cortex or NPY protein in the paraventricular nucleus. BAT UCP message levels were significantly higher (P = 0.001) in the HF group. Thus HF compared with HC and chow diet reduces expression of NPY mRNA in hypothalamic nuclei and increases expression of BAT UCP message.
我们使用不同饮食来测量饮食组成对下丘脑神经肽Y（NPY）信息和棕色脂肪解偶联蛋白（UCP）mRNA的影响。斯普拉格-道利大鼠随意进食普通饲料、高碳水化合物（HC）、中等碳水化合物（IHC）、高脂肪（HF）或中等脂肪（IHF）饮食，所有这些饮食的蛋白质含量（克/千卡）均相等。HF组和IHF组进食的食物量较少，并且在研究期间，除了HC组外，所有组消耗的千卡热量相似。1周后，我们处死动物，并从弓状核、皮质和棕色脂肪组织（BAT）中提取总RNA。HF组的弓状核NPY mRNA显著低于HC组和普通饲料组（P<0.001）。皮质中的NPY信息或室旁核中的NPY蛋白在各组之间没有差异。HF组的BAT UCP信息水平显著更高（P = 0.001）。因此，与HC和普通饲料饮食相比，HF饮食可降低下丘脑核中NPY mRNA的表达，并增加BAT UCP信息的表达。
