Taverne J, Reichlin M, Turk J L, Rees R J
Clin Exp Immunol. 1976 Apr;24(1):157-67.
A specific binding test was used to detect immune complexes containing antigens of Mycobacterium lepraemurium in the serum and tissues of infected mice. Complexes were precipitated by antiserum against immunoglogulin, free antigen removed by washing and the presence of bound antigen demonstrated by measurement of uptake of radioactively labelled specific antibody by the precipitate. Tests were done both with 125I-labelled FAB prepared from an immune from rabbit antiserum against M. Lepraemurium and with 125I-labelled IgG precipitate. Out of seventy-nine serum samples taken monthly up to the 5th month after infection, only there were positive (one at 2 months and two at 3 months). Kidneys taken from infected mice were also examined for immune complexes. Although deposits of IgM and sometimes of IgG were observed by immunoflourescence in glomeruli of normal mice, deposits of IgG were more frequent later on in infected mice. Nevertheless, binding tests done on acid eluates were positive in only one out of fifty-three infected mice.
采用特异性结合试验检测感染小鼠血清和组织中含鼠麻风杆菌抗原的免疫复合物。用抗免疫球蛋白抗血清沉淀复合物，通过洗涤去除游离抗原，通过测量沉淀物对放射性标记特异性抗体的摄取来证明结合抗原的存在。分别用从兔抗鼠麻风杆菌抗血清免疫制备的125I标记FAB和125I标记IgG沉淀物进行试验。在感染后第5个月每月采集的79份血清样本中，只有3份呈阳性（2个月时1份，3个月时2份）。还对感染小鼠的肾脏进行了免疫复合物检查。虽然在正常小鼠肾小球中通过免疫荧光观察到IgM沉积，有时也观察到IgG沉积，但在感染小鼠中，IgG沉积后来更频繁。然而，对酸洗脱液进行的结合试验在53只感染小鼠中只有1只呈阳性。
