Motoyama T, Watanabe H, Kumanishi T, Usui H, Hashimoto H, Hachisu T, Fukamachi I, Arai T, Takehara K
Department of Pathology, Niigata University School of Medicine, Japan.
Virchows Arch. 1994;424(4):361-6. doi: 10.1007/BF00190557.
We established a clonal cell line, HAT.MC8, derived from a human pulmonary large cell carcinoma with sarcomatoid features. This cell line was successfully maintained in a protein-free medium and exhibited sarcomatoid fibroblastic features in vitro. The cells constitutively produced a large amount of interleukin 6 (IL-6) in vitro. Tumour necrosis factor alpha (TNF-alpha) not only stimulated HAT.MC8 cells to produce IL-6, but also induced a morphological change from sarcomatoid fibroblastic to epithelial features. Although this change was related to actin and zonula adherens, there was no evidence that E-cadherin participated in the change. Interleukin 1 beta (IL-1 beta) had a stimulatory effect on IL-6 production by HAT.MC8 cells, but no influence on the morphology of the cells.
我们建立了一种克隆细胞系HAT.MC8，它源自具有肉瘤样特征的人肺大细胞癌。该细胞系在无蛋白培养基中成功维持，并在体外表现出肉瘤样成纤维细胞特征。这些细胞在体外组成性地产生大量白细胞介素6（IL-6）。肿瘤坏死因子α（TNF-α）不仅刺激HAT.MC8细胞产生IL-6，还诱导细胞形态从肉瘤样成纤维细胞特征转变为上皮特征。尽管这种变化与肌动蛋白和黏着小带有关，但没有证据表明E-钙黏蛋白参与了这种变化。白细胞介素1β（IL-1β）对HAT.MC8细胞产生IL-6有刺激作用，但对细胞形态没有影响。
