Coutelier J P, Godfraind C, Dveksler G S, Wysocka M, Cardellichio C B, Noël H, Holmes K V
Unit of Experimental Medicine, International Institute of Cellular and Molecular Pathology, St-Luc Hospital, Catholic University of Louvain, Brussels, Belgium.
Eur J Immunol. 1994 Jun;24(6):1383-90. doi: 10.1002/eji.1830240622.
The expression of carcinoembryonic antigen (CEA)-related glycoproteins that have been associated with intercellular adhesion and that serve as receptors for mouse hepatitis virus (MHV) was analyzed in cells from the immune system of BALB/c mice using immunolabeling and RNA polymerase chain reaction amplification of receptor transcripts. These glycoproteins, which are called biliary glycoproteins, were highly expressed in B lymphocytes, including cells of the B-1a (CD5+) lineage, and in macrophages, but were not detectable in resting T lymphocytes. Similarly, murine cell lines of B cell and macrophage origin expressed messenger RNA encoding CEA-related molecules, while the corresponding mRNA was only slightly detectable in a T cell line. These CEA-related cell adhesion glycoproteins were also expressed in endothelial cells. Therefore, their specific interaction with their so far unknown ligand may be of functional importance in cellular interactions in the immune response. Monoclonal antibody directed against these glycoproteins blocked MHV-A59 infection of the B cell-derived SP20 cell line. Thus, the functional receptors for MHV on B lymphocytes, like those on murine fibroblasts, are isoforms of CEA-related glycoproteins. Treatment of B cells with anti-receptor antibody also blocked B cell-mediated cytotoxicity against MHV-A59-infected fibroblasts, indicating that this phenomenon is mediated by interaction of viral attachment protein on the infected target cells with specific CEA-related receptor glycoproteins on the effector B cells.
利用免疫标记和受体转录本的RNA聚合酶链反应扩增，对BALB/c小鼠免疫系统细胞中与细胞间黏附相关且作为小鼠肝炎病毒（MHV）受体的癌胚抗原（CEA）相关糖蛋白的表达进行了分析。这些被称为胆汁糖蛋白的糖蛋白在B淋巴细胞（包括B-1a（CD5+）谱系的细胞）和巨噬细胞中高度表达，但在静息T淋巴细胞中未检测到。同样，B细胞和巨噬细胞来源的小鼠细胞系表达编码CEA相关分子的信使RNA，而在T细胞系中仅能轻微检测到相应的mRNA。这些CEA相关的细胞黏附糖蛋白也在内皮细胞中表达。因此，它们与目前未知配体的特异性相互作用可能在免疫应答中的细胞相互作用中具有重要功能。针对这些糖蛋白的单克隆抗体可阻断B细胞来源的SP20细胞系的MHV-A59感染。因此，B淋巴细胞上的MHV功能性受体与小鼠成纤维细胞上的受体一样，是CEA相关糖蛋白的异构体。用抗受体抗体处理B细胞也可阻断B细胞介导的对MHV-A59感染的成纤维细胞的细胞毒性，这表明该现象是由感染靶细胞上的病毒附着蛋白与效应B细胞上特定的CEA相关受体糖蛋白相互作用介导的。
