环磷酰胺对小鼠铜绿假单胞菌免疫反应的影响。
Effect of cyclophosphamide on the immune response to Pseudomonas aeruginosa in mice.
作者信息
Pierson C L, Johnson A G, Feller I
出版信息
Infect Immun. 1976 Jul;14(1):168-77. doi: 10.1128/iai.14.1.168-177.1976.
Natural resistance in mice to Pseudomonas aeruginosa was decreased 10-fold with a single dose of 300 mg of cyclophosphamide (CY) per kg intraperitoneally. Mice were resistant to infection when immunized actively with Pseudomonas vaccine or passively with Pseudomonas immune serum before receiving CY. Syngeneic spleen, thymus and/or bone marrow cells were transfused into CY-treated recipient mice. Protective anti-Pseudomonas antibody was elicited in the recipient mice when they were vaccinated 1 day after receiving normal spleen cells and challenged 8 days after vaccination. When 1.6 X 10(7) normal thymus and bone marrow cells were infused before vaccination, 69% of the recipients of both cell preparations responded serologically compared with 15 and 27% of those receiving either thymus or bone marrow cells, respectively. CY-treated thymus or bone marrow cell recipients were resistant to Pseudomonas infection when 6 X 10(7) of either cell population was transfused.
小鼠对铜绿假单胞菌的天然抵抗力在腹腔注射每千克300毫克环磷酰胺（CY）单剂量后降低了10倍。在接受CY之前，用铜绿假单胞菌疫苗主动免疫或用铜绿假单胞菌免疫血清被动免疫的小鼠对感染具有抵抗力。将同基因脾细胞、胸腺细胞和/或骨髓细胞输注到经CY处理的受体小鼠体内。当受体小鼠在接受正常脾细胞1天后接种疫苗，并在接种疫苗8天后受到攻击时，在受体小鼠中引发了保护性抗铜绿假单胞菌抗体。当在接种疫苗前注入1.6×10⁷个正常胸腺细胞和骨髓细胞时，两种细胞制剂的受体中有69%产生了血清学反应，而接受胸腺细胞或骨髓细胞的受体分别为15%和27%。当输注6×10⁷个上述任何一种细胞群体时，经CY处理的胸腺或骨髓细胞受体对铜绿假单胞菌感染具有抵抗力。
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