Jeejeebhoy H F
J Exp Med. 1970 Nov;132(5):963-75. doi: 10.1084/jem.132.5.963.
When CY-treated mice were given sheep red blood cells the serum hemagglutinin titers produced were significantly lower than those found when mice received SRBC but not CY. Titers could be raised to the levels found in the latter group if, in addition to SRBC, the CY-treated mice received 2 x 10(7) normal syngeneic spleen cells or a mixture containing 1.2 x 10(8) normal thymus and 1.2 x 10(8) normal marrow cells. Inocula which contained fewer cells produced correspondingly smaller amounts of antibody. A synergistic interaction between normal thymus and marrow cells was always demonstrable in these experiments. Hemagglutinin titers produced by CY-treated mice given SRBC and 2 x 10(7) normal syngeneic spleen cells were always much higher than those found when the spleen cells were obtained from animals previously given ALS. Titers could be raised to normal levels if the animals in this latter group received additional injections containing mixtures of normal syngeneic thymus and marrow cells. Marrow cells alone were completely ineffective, while inocula which only contained thymus cells were much less effective than mixtures of thymus and marrow cells. These results suggest that immunosuppression by ALS is associated with the inactivation of both thymus and marrow-derived lymphocytes. In other experiments CY-treated mice received SRBC and mixtures of thymus and marrow cells from both untreated and ALS-treated donors. No decrease in the immunological competence of cells located within the thymus of ALS-treated donors was demonstrable in these experiments. Marrow cells were slightly affected but to a markedly lesser degree than were spleen cells of ALS-treated animals. In a final experiment, it was possible to show that the thymus and marrow cells of ALS-treated animals could repair the immunological defects which were present in their own spleen cell populations.
当给经环磷酰胺（CY）处理的小鼠注射绵羊红细胞时，所产生的血清血凝素滴度显著低于未接受CY而仅接受绵羊红细胞（SRBC）的小鼠。如果除了SRBC之外，经CY处理的小鼠还接受2×10⁷个同基因正常脾细胞或含有1.2×10⁸个正常胸腺细胞和1.2×10⁸个正常骨髓细胞的混合物，则滴度可提高到后一组小鼠的水平。细胞数量较少的接种物产生的抗体量相应较少。在这些实验中，正常胸腺细胞和骨髓细胞之间的协同相互作用总是可以证明的。给经CY处理的小鼠注射SRBC和2×10⁷个同基因正常脾细胞所产生的血凝素滴度，总是远高于从先前接受过抗淋巴细胞血清（ALS）的动物获得的脾细胞所产生的滴度。如果后一组动物接受额外的含有同基因正常胸腺细胞和骨髓细胞混合物的注射，滴度可提高到正常水平。单独的骨髓细胞完全无效，而仅含胸腺细胞的接种物比胸腺细胞和骨髓细胞的混合物效果差得多。这些结果表明，ALS诱导的免疫抑制与胸腺来源和骨髓来源的淋巴细胞失活有关。在其他实验中，经CY处理的小鼠接受SRBC以及来自未处理和ALS处理供体的胸腺细胞和骨髓细胞混合物。在这些实验中，未证明ALS处理供体胸腺内细胞的免疫能力有所下降。骨髓细胞受到轻微影响，但程度明显低于ALS处理动物的脾细胞。在最后一个实验中，有可能证明ALS处理动物的胸腺细胞和骨髓细胞能够修复其自身脾细胞群体中存在的免疫缺陷。
