Armbruster D A, Schwarzhoff R H, Pierce B L, Hubster E C
Armstrong Laboratory Drug Testing Division, Brooks AFB, TX 78235-5000.
J Anal Toxicol. 1994 Mar-Apr;18(2):110-7. doi: 10.1093/jat/18.2.110.
The EMIT 700 and EMIT II assays for marijuana, cocaine, opiates, barbiturates, and phencyclidine, as performed on a Hitachi 717 Analyzer, were compared with Roche Abuscreen RIA tests for high-volume drug abuse screening. The EMIT II kits offer some advantages over the EMIT 700 kits for testing large work loads. The EMIT II marijuana test in particular exhibits a calibration curve that promises improved ability to separate negative from positive samples due to the increased absorbance rate separation between the negative and cutoff calibrators. Both EMIT formulations are preferable in terms of speed and ease of analysis in comparison with our laboratory's current RIA procedures. Over 50,000 urine samples were screened by EMIT 700 and RIA and by EMIT II and RIA. The performance of both EMIT assays was approximately equivalent to RIA in terms of their ability to detect urine samples that confirmed positive for cocaine and opiates. Both EMIT assays detected approximately 90% of the urine samples screened positive by RIA and confirmed positive for marijuana. Both EMIT assays performed better than RIA in detecting confirmed-positive barbiturate samples.
在日立717分析仪上进行的用于检测大麻、可卡因、阿片类药物、巴比妥类药物和苯环己哌啶的EMIT 700和EMIT II检测方法，与用于大批量药物滥用筛查的罗氏Abuscreen RIA检测进行了比较。在检测大量工作量时，EMIT II试剂盒相对于EMIT 700试剂盒具有一些优势。特别是EMIT II大麻检测呈现出一条校准曲线，由于阴性校准品和临界值校准品之间吸光度率分离的增加，有望提高区分阴性和阳性样本的能力。与我们实验室目前的RIA程序相比，两种EMIT方法在分析速度和简便性方面都更可取。通过EMIT 700和RIA以及EMIT II和RIA对超过50000份尿液样本进行了筛查。就检测可卡因和阿片类药物确认阳性的尿液样本的能力而言，两种EMIT检测的性能与RIA大致相当。两种EMIT检测都检测出了约90%经RIA筛查呈阳性且大麻确认阳性的尿液样本。在检测确认阳性的巴比妥类药物样本方面，两种EMIT检测都比RIA表现更好。
