Shirato I, Sakai T, Fukui M, Tomino Y, Koide H
Department of Medicine, Juntendo University, School of Medicine, Tokyo, Japan.
Virchows Arch A Pathol Anat Histopathol. 1993;423(2):121-9. doi: 10.1007/BF01606586.
Morphological and morphometric studies of glomeruli were carried out in streptozotocin-induced diabetic rats using improved tissue processing and computerized morphometry. Increased mesangial matrix, occupying the enlarged diabetic mesangium, contained an abundance of dark granular material in addition to the microfibrils which were usually found in the control glomeruli. In the diabetic glomeruli, the lamina densa was thick and heterogeneous showing a dense layer both on its epithelial and endothelial aspects, and the lamina rara externa contained more fibrils than in control rats. Detailed estimation of the absolute values of the various compartments of the diabetic glomeruli by using perfusion-fixed materials and a computer-assisted digitizer revealed that the volume and surface area of the mesangium were increased more extensively than those of the capillary; the enlargement of the mesangial-capillary interface area was the most pronounced among the morphometric changes of the diabetic glomeruli; and that the moderate increase in capillary volume was associated with an increased radius. Our quantitative results showed that capillaries in the diabetic glomeruli had an extensively wider neck which may be the first sign of structural damage to the glomerular tuft.
利用改进的组织处理方法和计算机形态测量技术，对链脲佐菌素诱导的糖尿病大鼠的肾小球进行了形态学和形态测量学研究。糖尿病大鼠肾小球系膜基质增加，占据扩大的系膜区，除了对照肾小球中常见的微纤维外，还含有大量深色颗粒物质。在糖尿病肾小球中，致密层增厚且不均一，在其上皮侧和内皮侧均显示出致密层，且外侧稀疏层比对照大鼠含有更多的纤维。通过使用灌注固定材料和计算机辅助数字化仪对糖尿病肾小球各部分的绝对值进行详细估计，结果显示，系膜的体积和表面积比毛细血管增加得更广泛；系膜 - 毛细血管界面面积的扩大是糖尿病肾小球形态测量变化中最明显的；毛细血管体积的适度增加与半径增加有关。我们的定量结果表明，糖尿病肾小球中的毛细血管颈部广泛增宽，这可能是肾小球毛细血管丛结构损伤的首个迹象。
