Mary C, Ange G, Dunan S, Lamouroux D, Quilici M
Laboratoire de Parasitologie, Faculte de Medecine, Marseille, France.
Am J Trop Med Hyg. 1993 Oct;49(4):492-501. doi: 10.4269/ajtmh.1993.49.492.
Complications of visceral leishmaniasis are explained in part by immune complex pathology, particularly nephritis. Using the polyethylene glycol precipitation method, we identified among these complexes a Leishmania infantum circulating antigen by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. Monoclonal antibodies were produced to specifically detect this antigen. This protein (molecular weight 51 kD) is a cytoplasmic antigen of L. infantum and its synthesis by promastigotes is related to stationary-phase culture. It was present in sera from dogs whose leishmaniasis was associated with nephritis, and it was found in the sera of 30% of human patients with visceral leishmaniasis. It was also detected in the sera of patients with cutaneous leishmaniasis, and it persists in the sera of clinically cured subjects.
内脏利什曼病的并发症部分是由免疫复合物病理学解释的，尤其是肾炎。我们使用聚乙二醇沉淀法，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和免疫印迹，在这些复合物中鉴定出一种婴儿利什曼原虫循环抗原。制备了单克隆抗体以特异性检测该抗原。这种蛋白质（分子量51kD）是婴儿利什曼原虫的一种细胞质抗原，其前鞭毛体的合成与稳定期培养有关。它存在于利什曼病合并肾炎的犬的血清中，并且在30%的内脏利什曼病患者血清中也被发现。它也在皮肤利什曼病患者的血清中被检测到，并且在临床治愈的受试者血清中持续存在。
