一种新型枯草芽孢杆菌生产杆菌肽。提取、纯化及特性鉴定。
Bacitracin production by a new strain of Bacillus subtilis. Extraction, purification, and characterization.
作者信息
Azevedo E C, Rios E M, Fukushima K, Campos-Takaki G M
机构信息
Mestrado de Ciencias Farmacêuticas, Universidade Federal de Pernambuco, Recife-PE, Brazil.
出版信息
Appl Biochem Biotechnol. 1993 Jul;42(1):1-7. doi: 10.1007/BF02788897.
A new strain of Bacillus subtilis C 126 was isolated from sugar cane fermentation and produced an antibiotic that inhibited the growth of Micrococcus flavus. The production of the antibiotic in culture medium followed to extraction with n-butanol, thin layer chromatography, and microbiological tests indicated that a polypeptide antibiotic was produced. The fraction obtained by Sephadex G-25 column and analyzed by HPLC indicated that bacitracin complex was produced.
从甘蔗发酵物中分离出一种新型枯草芽孢杆菌C 126，它产生了一种能抑制黄色微球菌生长的抗生素。在培养基中生产该抗生素后，经正丁醇萃取、薄层色谱法和微生物测试表明产生了一种多肽抗生素。通过葡聚糖G - 25柱获得的馏分经高效液相色谱分析表明产生了杆菌肽复合物。
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