急性体内和体外暴露于烟草烟雾对小鼠肺泡巨噬细胞肿瘤坏死因子α产生的抑制作用。

Inhibition of mouse alveolar macrophage production of tumor necrosis factor alpha by acute in vivo and in vitro exposure to tobacco smoke.

作者信息

Higashimoto Y, Shimada Y, Fukuchi Y, Ishida K, Shu C, Teramoto S, Sudo E, Matsuse T, Orimo H

机构信息

Department of Geriatrics, Faculty of Medicine, University of Tokyo, Japan.

出版信息

Respiration. 1992;59(2):77-80. doi: 10.1159/000196031.

DOI:10.1159/000196031

PMID:1620985

Abstract

We investigated the effects of tobacco smoke exposure on the production of tumor necrosis factor alpha (TNF alpha) by alveolar macrophages (AM) in mice (C57BL/6). The results obtained are as follows: (1) In vivo tobacco smoke exposure caused a significant decrease in the production of TNF alpha by AM with the stimulation of lipopolysaccharide (LPS; control group: 19.32 +/- 5.52 U/ml, smoked group: 4.28 +/- 0.98 U/ml; p less than 0.05). (2) In vitro exposure of AM to tobacco smoke extracts (water-soluble extracts) also caused a decrease in the production of TNF alpha up to 93% of control with stimulation of LPS (p less than 0.05) without any decrease in cellular viability. We concluded that the production of TNF alpha by AM was impaired by smoking via direct action of the factors present in tobacco smoke.

摘要

我们研究了烟草烟雾暴露对小鼠（C57BL/6）肺泡巨噬细胞（AM）产生肿瘤坏死因子α（TNFα）的影响。获得的结果如下：（1）在体内，烟草烟雾暴露导致经脂多糖（LPS）刺激后AM产生TNFα的量显著降低（对照组：19.32±5.52 U/ml，吸烟组：4.28±0.98 U/ml；p<0.05）。（2）在体外，将AM暴露于烟草烟雾提取物（水溶性提取物）也导致经LPS刺激后TNFα的产生减少，最多可达对照组的93%（p<0.05），而细胞活力没有任何降低。我们得出结论，吸烟通过烟草烟雾中存在的因子的直接作用损害了AM产生TNFα的能力。