短小芽孢杆菌中的选择性质粒转导
Selective plasmid transduction in Bacillus pumilus.
作者信息
Bramucci M G, Lovett P S
出版信息
J Bacteriol. 1977 Sep;131(3):1029-32. doi: 10.1128/jb.131.3.1029-1032.1977.
Abstract
The inducible temperate bacteriophage phi75 and a clear-plaque-forming variant, phi75C1, mediated transduction of a 4.4 X 10(6)-dalton multicopy Bacillus pumilus plasmid, pPL10, at frequencies of 10(-5) to 10(-6) transductants per plaque-forming unit. phi75- and phi75C1-mediated transduction of several chromosome markers tested did not occur at a detectable frequency. phi75-mediated plasmid transducing activity resides in particles that are similar to infectious particles in sedimentation velocity and buoyant density.
摘要
可诱导的温和噬菌体phi75和一个形成清晰噬菌斑的变体phi75C1介导了4.4×10⁶道尔顿多拷贝短小芽孢杆菌质粒pPL10的转导，转导频率为每噬菌斑形成单位10⁻⁵至10⁻⁶个转导子。phi75和phi75C1介导的几种测试染色体标记的转导未在可检测频率下发生。phi75介导的质粒转导活性存在于沉降速度和浮力密度与感染性颗粒相似的颗粒中。
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