Murphy L C, Dotzlaw H, Hamerton J, Schwarz J
Department of Biochemistry, University of Manitoba, Winnipeg, Canada.
Breast Cancer Res Treat. 1993;26(2):149-61. doi: 10.1007/BF00689688.
Variant, smaller sized, estrogen receptor-like mRNAs have been previously cloned and sequenced from human breast cancer biopsy samples. In this study we have found that one variant estrogen receptor-like mRNA, clone 24, which is expressed in only one tumor, consists of sequences from the normal ER gene which is found on chromosome 6 and sequences unrelated to the ER which are found normally on chromosome 12. A more commonly occurring variant estrogen receptor-like mRNA, clone 4, contains sequences from the normal ER gene and LINE-1 sequences. Similar but not identical LINE-1 sequences have been found in some of the intervening sequences of the normal ER gene. The generation of these transcripts may involve either alternative splicing or trans-splicing mechanisms.
之前已从人类乳腺癌活检样本中克隆并测序出了变异的、较小尺寸的雌激素受体样mRNA。在本研究中，我们发现一种变异的雌激素受体样mRNA（克隆24）仅在一个肿瘤中表达，它由位于6号染色体上的正常雌激素受体（ER）基因序列以及通常位于12号染色体上与ER无关的序列组成。一种更常见的变异雌激素受体样mRNA（克隆4）包含正常ER基因序列和LINE-1序列。在正常ER基因的一些间隔序列中也发现了相似但不完全相同的LINE-1序列。这些转录本的产生可能涉及选择性剪接或反式剪接机制。
