McGuire J C, Pène J J, Barrow-Carraway J
J Virol. 1974 Mar;13(3):690-8. doi: 10.1128/JVI.13.3.690-698.1974.
Fifty-four suppressible mutants of bacteriophage phi29 have been isolated with a variety of mutagens and assigned to eight complementation groups. Viral-specific protein synthesis in UV light-irradiated, nonsuppressing Bacillus subtilis 60084 was analyzed with exponential acrylamide gels. Four additional phi29 proteins which were undetected on ordinary acrylamide gels are reported in this paper. Five phage phi29 proteins have been unambiguously assigned to specific cistrons. Two cistrons had pleiotropic effects on viral protein synthesis. Mutants in cistrons I or II were unable to synthesize DNA in nonsuppressing bacteria. Mutants in cistron I were unable to attach viral chromosomes to the host cell membrane, and the protein responsible for this function has been identified. The other viral protein playing a role in phage phi29 DNA synthesis is also identified and assigned to cistron II. Mutants in cistron II can attach viral chromosomes to membrane, but cannot synthesize DNA in nonsuppressing bacteria.
利用多种诱变剂已分离出54株噬菌体φ29的可抑制突变体，并将其归入8个互补群。用指数聚丙烯酰胺凝胶分析了紫外线照射的、非抑制性枯草芽孢杆菌60084中病毒特异性蛋白质的合成。本文报道了在普通聚丙烯酰胺凝胶上未检测到的另外4种φ29蛋白。5种噬菌体φ29蛋白已明确归属于特定的顺反子。两个顺反子对病毒蛋白质合成有多效性作用。顺反子I或II中的突变体在非抑制性细菌中不能合成DNA。顺反子I中的突变体不能将病毒染色体附着于宿主细胞膜，并且已鉴定出负责此功能的蛋白质。还鉴定出了在噬菌体φ29 DNA合成中起作用的另一种病毒蛋白，并将其归入顺反子II。顺反子II中的突变体可以将病毒染色体附着于膜，但在非抑制性细菌中不能合成DNA。
