定位到大鼠11号染色体的简单序列重复长度多态性

Simple sequence repeat length polymorphisms mapped to rat chromosome 11.

作者信息

Du Y, Remmers E F, Goldmuntz E A, Zha H, Mathern P, Crofford L J, Wilder R L

机构信息

Arthritis and Rheumatism Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD.

出版信息

Cytogenet Cell Genet. 1994;65(3):186-9. doi: 10.1159/000133629.

DOI:10.1159/000133629

PMID:8222758

Abstract

Two genes and two anonymous DNA loci were mapped to rat chromosome 11 using F2 intercross progeny of Fischer (F344/N) and Lewis (LEW/N) inbred rats. These four loci formed a single linkage group covering 21.5 cM with the following map order: somatostatin (SST)-D11N161-D11N18-cell surface protein (MOX2). These four loci were typed by PCR-based simple sequence repeat (SSR) length polymorphism detection. For each marker four to seven different alleles were detected using a panel of 13 inbred rat strains (F344/N, LEW/N, BN/SsN, BUF/N, LER/N, MR/N, MNR/N, LOU/MN, ACI/N, WBB1/N, WBB2/N, SHR/N, WKY/N). Comparative gene mapping analysis suggests syntenic conservation between rat chromosome 11 and mouse Chromosome 16.

摘要

利用Fischer（F344/N）和Lewis（LEW/N）近交系大鼠的F2代杂交后代，将两个基因和两个无名DNA位点定位到大鼠的11号染色体上。这四个位点形成了一个单一的连锁群，覆盖21.5厘摩，其图谱顺序如下：生长抑素（SST）-D11N161-D11N18-细胞表面蛋白（MOX2）。通过基于聚合酶链反应（PCR）的简单序列重复（SSR）长度多态性检测对这四个位点进行分型。使用一组13个近交系大鼠品系（F344/N、LEW/N、BN/SsN、BUF/N、LER/N、MR/N、MNR/N、LOU/MN、ACI/N、WBB1/N、WBB2/N、SHR/N、WKY/N）检测到每个标记有4至7个不同的等位基因。比较基因定位分析表明大鼠11号染色体与小鼠16号染色体之间存在同线保守性。