Mathern P, Goldmuntz E A, Zha H, Du Y, Crofford L J, Wilder R L, Remmers E F
Arthritis and Rheumatism Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Biochem Genet. 1993 Oct;31(9-10):441-8. doi: 10.1007/BF02396228.
Four PCR-typable polymorphic markers were mapped to rat chromosome 12 by linkage analysis of F2 intercross progeny of Fischer (F344/N) and Lewis (LEW/N) rat strains. The markers formed a single linkage group, covering 27.7 cM, with the following order and distance between markers: plasminogen activator inhibitor (Planh)--0.0 cM--phosphoenolpyruvate carboxykinase-related sequence 2 (Pepckr2)--15.4 cM--anonymous marker (D12N155)--12.3 cM--serine dehydratase (Sdh). All markers were identified and genotyped by PCR analysis of simple sequence repeats. The gene encoding Planh was previously assigned to rat chromosome 12, which allowed us to assign the entire linkage group to this chromosome. These markers were highly polymorphic in 13 additional inbred rat strains (BUF/N, BN/SsN, WKY/N, MNR/N, LER/N, WBB1/N, WBB2/N, MR/N, LOU/MN, SHR/N, ACI/N, SR/Jr, and SS/Jr). These markers should be useful tools for further genetic studies in rats.
通过对Fischer（F344/N）和Lewis（LEW/N）大鼠品系的F2代杂交后代进行连锁分析，将四个可通过聚合酶链反应（PCR）分型的多态性标记定位到大鼠第12号染色体上。这些标记形成了一个单一的连锁群，覆盖27.7厘摩（cM），标记之间的顺序和距离如下：纤溶酶原激活物抑制剂（Planh）——0.0厘摩——磷酸烯醇丙酮酸羧激酶相关序列2（Pepckr2）——15.4厘摩——匿名标记（D12N155）——12.3厘摩——丝氨酸脱水酶（Sdh）。所有标记均通过对简单序列重复序列进行PCR分析来鉴定和基因分型。编码Planh的基因先前已被定位到大鼠第12号染色体上，这使我们能够将整个连锁群定位到该染色体上。这些标记在另外13个近交大鼠品系（BUF/N、BN/SsN、WKY/N、MNR/N、LER/N、WBB1/N、WBB2/N、MR/N、LOU/MN、SHR/N、ACI/N、SR/Jr和SS/Jr）中具有高度多态性。这些标记应为大鼠进一步的遗传学研究提供有用的工具。
