Dawirs R R, Teuchert-Noodt G, Molthagen M
Department of Neuroanatomy, Faculty of Biology, University of Bielefeld, Germany.
Eur J Pharmacol. 1993 Sep 7;241(1):89-97. doi: 10.1016/0014-2999(93)90937-d.
A single dose of methamphetamine (25 mg/kg i.p.) was administered to young adult gerbils (Meriones unguiculatus) aged 90 days and the number of spices was determined along 40-microns segments of basal, lateral and apical dendrites of pyramidal cells in layers III and V of the prefrontal cortex, after 1.5, 7, 20 and 30 days. The density of spines rapidly increased by more than 80% within 7 days after drug challenge, and subsequently returned to the original normal values within about 2 weeks. Thirty days after drug administration the density of dendritic spines was slightly, but significantly, less than control values (about 5%). The density of spines was likewise affected in layer III and V neurones, irrespective of the spatial domain of their dendritic ramifications. Since several lines of investigation indicate that methamphetamine can cause the destruction of dopaminergic nerve terminals in the mammalian forebrain, the present results are discussed against the background of current concepts about reactive synaptic reorganization and adaptive remodelling of neural circuits in the central nervous system.
给90日龄的成年幼年沙鼠（长爪沙鼠）腹腔注射单剂量甲基苯丙胺（25毫克/千克），并在给药后1.5、7、20和30天，确定前额叶皮质第III层和第V层锥体细胞基底、外侧和顶端树突40微米节段上的棘突数量。药物激发后7天内，棘突密度迅速增加超过80%，随后在约2周内恢复到原始正常值。给药30天后，树突棘密度略有下降，但显著低于对照值（约5%）。第III层和第V层神经元的棘突密度同样受到影响，无论其树突分支的空间区域如何。由于多项研究表明甲基苯丙胺可导致哺乳动物前脑多巴胺能神经末梢的破坏，因此在当前关于中枢神经系统中反应性突触重组和神经回路适应性重塑概念的背景下讨论了本研究结果。
