Dawirs R R, Teuchert-Noodt G, Busse M
Department of Neuroanatomy, Faculty of Biology, University of Bielefeld, Germany.
Neuropharmacology. 1991 Mar;30(3):275-82. doi: 10.1016/0028-3908(91)90155-5.
Single doses of methamphetamine (25 mg/kg) were administered to adult gerbils. All detectable spines were counted along defined segments of basal, apical and lateral dendrites of Golgi-impregnated pyramidal cells in the medial prefrontal cortex (layers III and V) and the parietal cortex (layer V). These two areas were selected because previous investigations had shown methamphetamine-induced neurotoxicity in prefrontal cortex but not in parietal cortex. The frequencies of dendritic spines in methamphetamine-treated gerbils were found to be significantly increased in the prefrontal cortex, while no significant change was found in the parietal cortex.
给成年沙鼠单剂量注射甲基苯丙胺（25毫克/千克）。沿着内侧前额叶皮质（III层和V层）和顶叶皮质（V层）中经高尔基染色的锥体细胞的基底、顶端和侧枝树突的特定节段，对所有可检测到的棘突进行计数。选择这两个区域是因为先前的研究表明甲基苯丙胺会在前额叶皮质而非顶叶皮质中引起神经毒性。结果发现，经甲基苯丙胺处理的沙鼠前额叶皮质中树突棘的频率显著增加，而顶叶皮质中未发现显著变化。
