新型改良的lacZ基因融合载体。
New improved lacZ gene fusion vectors.
作者信息
Jain C
机构信息
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
出版信息
Gene. 1993 Oct 29;133(1):99-102. doi: 10.1016/0378-1119(93)90231-q.
New plasmid vectors suitable for creating fusions with the lacZ gene have been developed. These vectors represent an improvement over currently available vectors and possess the following features: (1) an undetectable background beta-galactosidase (beta Gal) activity in the absence of fusion, (2) an extended multiple cloning site (MCS), and (3) the ability to conveniently subclone in any one of three translational frames. Medium- and high-copy-number versions of these vectors have been developed.
已开发出适用于与lacZ基因构建融合体的新型质粒载体。这些载体是对现有载体的改进，具有以下特点：（1）在无融合的情况下背景β-半乳糖苷酶（βGal）活性不可检测；（2）具有扩展的多克隆位点（MCS）；（3）能够方便地在三个翻译框架中的任何一个中进行亚克隆。已开发出这些载体的中拷贝数和高拷贝数版本。
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