St Pierre R, Linn T
Department of Microbiology and Immunology, Faculty of Medicine, University of Western Ontario, London, Canada.
Gene. 1996 Feb 22;169(1):65-8. doi: 10.1016/0378-1119(95)00787-3.
New single-copy vectors based on lambda phage have been developed for creating either transcriptional (operon) or translational (gene) fusions to the lacZ gene. The improvements of these vectors over the previous lambda TL61 vector include: (i) incorporation of a tetracycline-resistance-encoding gene (TcR) to permit direct selection of lysogens, (ii) low-background beta-galactosidase activity, (iii) the ability to accept DNA inserts up to 8 kb in size, and (iv) an expanded multiple cloning site (MCS). The new transcriptional fusion vector retains the RNase III processing site downstream from the MCS which ensures independent translation of lacZ. The set of three translational fusion vectors allow for convenient subcloning in any of the three translational reading frames.
基于λ噬菌体的新型单拷贝载体已被开发出来，用于创建与lacZ基因的转录（操纵子）或翻译（基因）融合体。这些载体相对于先前的λTL61载体的改进包括：（i）并入四环素抗性编码基因（TcR）以允许直接选择溶原菌，（ii）低背景β-半乳糖苷酶活性，（iii）接受大小达8 kb的DNA插入片段的能力，以及（iv）扩展的多克隆位点（MCS）。新的转录融合载体在MCS下游保留了RNase III加工位点，这确保了lacZ的独立翻译。这一组三个翻译融合载体允许在三个翻译阅读框中的任何一个中方便地进行亚克隆。
