在尿沉渣分析期间，使用衣原体酶确认阶段为强制要求。
Mandatory use of confirmation stage with Chlamydiazyme during urinary sediment analysis.
作者信息
Anderson J R, Mumtaz G, Mulhare P, Poddar J, Ridgway G L
机构信息
Department of Clinical Microbiology, University College Hospital, London.
出版信息
J Clin Pathol. 1993 Oct;46(10):896-7. doi: 10.1136/jcp.46.10.896.
AIMS
To assess whether false positive results found when the first stage Chlamydiazyme test is performed on urinary sediment could be reduced by using the more specific second stage blocking assay.
METHODS
Sediment from 173 urine samples from patients with suspected urinary tract infection caused by Gram negative bacteria and 23 control urine samples were tested using the Chlamydiazyme assay system, which included a blocking assay.
RESULTS
A reaction result with the first stage Chlamydiazyme assay test was seen in 102 (58.9%) of the test urine samples. First stage reactivity was not blocked by the Chlamydiazyme confirmatory assay performed on repeat testing. All were correctly identified as true negative (first test false positive) results.
CONCLUSIONS
Use of a second (specific) blocking assay for the analysis of urinary sediment using Chlamydiazyme eliminates false positive results in Gram negative urinary tract infections.
目的
评估通过使用更具特异性的第二阶段阻断试验，能否减少在尿沉渣上进行第一阶段衣原体酶试验时出现的假阳性结果。
方法
使用包含阻断试验的衣原体酶检测系统，对173份疑似革兰氏阴性菌引起的尿路感染患者的尿液样本沉淀物以及23份对照尿液样本进行检测。
结果
在102份（58.9%）检测尿液样本中，第一阶段衣原体酶试验出现反应结果。重复检测时，第一阶段的反应性未被衣原体酶确证试验阻断。所有结果均被正确鉴定为真阴性（首次检测假阳性）结果。
结论
使用第二种（特异性）阻断试验对尿沉渣进行衣原体酶分析，可消除革兰氏阴性菌尿路感染中的假阳性结果。
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