使用商业PCR试剂盒检测沙眼衣原体。
Use of a commercial PCR kit for detecting Chlamydia trachomatis.
作者信息
Smith I W, Morrison C L, Patrizio C, McMillan A
机构信息
Department of Medical Microbiology, University of Edinburgh, Medical School.
出版信息
J Clin Pathol. 1993 Sep;46(9):822-5. doi: 10.1136/jcp.46.9.822.
AIMS
To evaluate a commercial polymerase chain reaction (PCR) kit for the detection of Chlamydia trachomatis.
METHODS
Two hundred and fifty seven genital specimens, which had been submitted in 2SP medium for chlamydial isolation and subsequently stored at -70 degrees C, were retrospectively examined by a commercial PCR kit which detects chlamydial plasmid DNA. Culture negative, PCR positive specimens were examined by immunofluorescence and an in-house major outer membrane protein (MOMP)-PCR.
RESULTS
All 49 specimens which were culture positive were also PCR positive. Another 14 specimens were also PCR positive. After resolution of these results by immunofluorescence and a PCR assay for MOMP the sensitivity for PCR was 98.4% and that of culture 79%. The specificities were 99.5% and 100%, respectively.
CONCLUSIONS
This kit, which is highly sensitive and specific, is straightforward to use and has a built-in safeguard against cross contamination. The role of this test in the examination of routine genital specimens from patients with uncomplicated chlamydial infection is questionable due to its expense. It may have a place in the investigation of trachoma or infertility, however, where it has been shown that DNA can be detected when culture is unsuccessful.
目的
评估一种用于检测沙眼衣原体的商用聚合酶链反应（PCR）试剂盒。
方法
回顾性分析257份生殖器标本，这些标本曾置于2SP培养基中用于衣原体分离，随后保存在-70℃。采用一种检测衣原体质粒DNA的商用PCR试剂盒对其进行检测。对培养阴性但PCR阳性的标本进行免疫荧光检测和内部主要外膜蛋白（MOMP）-PCR检测。
结果
所有49份培养阳性的标本PCR检测也呈阳性。另外14份标本PCR检测也呈阳性。经免疫荧光和MOMP-PCR检测对这些结果进行分析后，PCR的敏感性为98.4%，培养的敏感性为79%。特异性分别为99.5%和100%。
结论
该试剂盒高度敏感且特异，使用简便，具有防止交叉污染的内置防护措施。由于费用问题，该检测方法在无并发症衣原体感染患者的常规生殖器标本检查中的作用值得怀疑。然而，在沙眼或不孕症的调查中，当培养不成功时仍能检测到DNA，它可能有一定作用。
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