Mandrup S, Andreasen P H, Knudsen J, Kristiansen K
Institute of Biochemistry, University of Odense, Denmark.
Mol Cell Biochem. 1993;123(1-2):55-61. doi: 10.1007/BF01076475.
Acyl-CoA-Binding Protein (ACBP)/Diazepam-Binding Inhibitor (DBI) is a 10 kD protein which has been implicated in a surprisingly large number of biochemical functions. We have unambiguously demonstrated that ACBP binds acyl-CoA esters with high affinity and in vivo functions as an acyl-CoA ester pool former. We have molecularly cloned and characterized the rat ACBP gene family which comprises one expressed and four processed pseudogenes. One of these was shown to exist in two allelic forms. A comprehensive computer-aided analysis of the promoter region of the expressed ACBP gene revealed that it exhibits all the hallmarks of typical housekeeping genes. In addition, the promoter region harbors a number of potential tissue specific cis-acting elements that may in part regulate the level of ACBP expression in specialized cells.
酰基辅酶A结合蛋白（ACBP）/地西泮结合抑制剂（DBI）是一种10 kD的蛋白质，它涉及大量令人惊讶的生化功能。我们已经明确证明ACBP以高亲和力结合酰基辅酶A酯，并且在体内作为酰基辅酶A酯库形成剂发挥作用。我们已经对大鼠ACBP基因家族进行了分子克隆和表征，该家族包括一个表达基因和四个加工假基因。其中一个被证明以两种等位基因形式存在。对表达的ACBP基因启动子区域进行的全面计算机辅助分析表明，它具有典型管家基因的所有特征。此外，启动子区域含有一些潜在的组织特异性顺式作用元件，这些元件可能部分调节特殊细胞中ACBP的表达水平。
