化脓隐秘杆菌（放线菌）质粒pAP1是滚环复制质粒pIJ101/pJV1家族的成员。
The Arcanobacterium (Actinomyces) pyogenes plasmid pAP1 is a member of the pIJ101/pJV1 family of rolling circle replication plasmids.
作者信息
Billington S J, Jost B H, Songer J G
机构信息
Department of Veterinary Science and Microbiology, University of Arizona, Tucson 85721, USA.
出版信息
J Bacteriol. 1998 Jun;180(12):3233-6. doi: 10.1128/JB.180.12.3233-3236.1998.
Abstract
The 2.4-kb plasmid pAP1 from Arcanobacterium (Actinomyces) pyogenes had sequence similarity within the putative replication protein and double-stranded origin with the pIJ101/pJV1 family of plasmids. pJGS84, a derivative of pAP1 containing a kanamycin resistance gene, was able to replicate in Escherichia coli and Corynebacterium pseudotuberculosis, as well as in A. pyogenes. Detection of single-stranded DNA intermediates of pJGS84 replication suggested that this plasmid replicates by the rolling circle mechanism.
摘要
来自化脓隐秘杆菌（放线菌属）的2.4kb质粒pAP1在假定的复制蛋白和双链原点区域与pIJ101/pJV1质粒家族具有序列相似性。pJGS84是pAP1的衍生物，含有卡那霉素抗性基因，能够在大肠杆菌、伪结核棒状杆菌以及化脓隐秘杆菌中复制。对pJGS84复制过程中单链DNA中间体的检测表明，该质粒通过滚环机制进行复制。
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