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化脓隐秘杆菌（放线菌）质粒pAP1是滚环复制质粒pIJ101/pJV1家族的成员。

The Arcanobacterium (Actinomyces) pyogenes plasmid pAP1 is a member of the pIJ101/pJV1 family of rolling circle replication plasmids.

作者信息

Billington S J, Jost B H, Songer J G

机构信息

Department of Veterinary Science and Microbiology, University of Arizona, Tucson 85721, USA.

出版信息

J Bacteriol. 1998 Jun;180(12):3233-6. doi: 10.1128/JB.180.12.3233-3236.1998.

DOI:10.1128/JB.180.12.3233-3236.1998
PMID:9620977
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC107828/
Abstract

The 2.4-kb plasmid pAP1 from Arcanobacterium (Actinomyces) pyogenes had sequence similarity within the putative replication protein and double-stranded origin with the pIJ101/pJV1 family of plasmids. pJGS84, a derivative of pAP1 containing a kanamycin resistance gene, was able to replicate in Escherichia coli and Corynebacterium pseudotuberculosis, as well as in A. pyogenes. Detection of single-stranded DNA intermediates of pJGS84 replication suggested that this plasmid replicates by the rolling circle mechanism.

摘要

来自化脓隐秘杆菌（放线菌属）的2.4kb质粒pAP1在假定的复制蛋白和双链原点区域与pIJ101/pJV1质粒家族具有序列相似性。pJGS84是pAP1的衍生物，含有卡那霉素抗性基因，能够在大肠杆菌、伪结核棒状杆菌以及化脓隐秘杆菌中复制。对pJGS84复制过程中单链DNA中间体的检测表明，该质粒通过滚环机制进行复制。

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本文引用的文献

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Rolling-circle replication of bacterial plasmids.细菌质粒的滚环复制
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Electroporation-mediated transformation of Arcanobacterium (Actinomyces) pyogenes.电穿孔介导的化脓隐秘杆菌（放线菌属）转化
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The Arcanobacterium (Actinomyces) pyogenes hemolysin, pyolysin, is a novel member of the thiol-activated cytolysin family.化脓隐秘杆菌（放线菌属）溶血素——化脓素，是硫醇激活溶血素家族的一个新成员。
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Nucleotide sequence of a nicking site of the Streptomyces plasmid pSN22 replicating by the rolling circle mechanism.通过滚环机制复制的链霉菌质粒pSN22切口位点的核苷酸序列。
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6
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