Stack M J, Scott A C, Done S H, Dawson M
Central Veterinary Laboratory, New Haw, Addlestone, Surrey.
Res Vet Sci. 1993 Sep;55(2):173-8. doi: 10.1016/0034-5288(93)90077-s.
Samples of cerebral cortex from eight scrapie affected sheep and two unaffected control sheep were stored for up to nine days at temperatures ranging from 18 degrees C to 29 degrees C. Scrapie associated fibrils (SAF) could be detected in proteinase K treated brain extracts from all the eight scrapie affected animals after five days storage and in six out of the eight after nine days storage. SAF could not be detected in any brain extracts from the two control animals. Formol saline fixed brain material from a further six scrapie affected and two clinically normal sheep, were also subjected to an extraction technique used to detect fibrils. No characteristic SAF were observed in any of these fixed samples. Long filamentous structures were observed in four of the fixed scrapie affected brain extracts and in one of the fixed unaffected control brain extracts.
取自8只患羊瘙痒症绵羊和2只未受影响对照绵羊的大脑皮层样本，在18摄氏度至29摄氏度的温度下保存长达9天。在储存5天后，从所有8只患羊瘙痒症的动物经蛋白酶K处理的脑提取物中均可检测到羊瘙痒症相关纤维（SAF），在储存9天后，8只中有6只可检测到。在2只对照动物的任何脑提取物中均未检测到SAF。另外6只患羊瘙痒症和2只临床正常绵羊的经甲醛生理盐水固定的脑材料，也采用用于检测纤维的提取技术进行处理。在这些固定样本中均未观察到特征性的SAF。在4份固定的患羊瘙痒症脑提取物和1份固定的未受影响对照脑提取物中观察到了长丝状结构。
