Freeman W R, Schneiderman T E, Wiley C A, Listhaus A D, Svendsen P, Munguia D, Bergeron-Lynn G
Department of Ophthalmology, University of California, San Diego 92093.
Retina. 1993;13(3):214-21. doi: 10.1097/00006982-199313030-00005.
To study local intravitreal therapies for retinitis due to herpes viruses, an animal model of focal, subacute, relatively nonlethal herpes family retinitis is needed. Herpes simplex virus type 1 (HSV-1) was injected into the subretinal space of 33 Dutch pigmented rabbit eyes. The animals were observed for up to 42 days after the inoculation. All inoculated eyes developed a focal, enlarging area of retinitis in a predictable manner, showing focal enlarging areas of retinal opacification and necrosis with variable retinal hemorrhage. In the inoculated eyes, retinal detachment developed in all animals within 21 days; 33% of the animals developed focal retinitis in the uninoculated eye. Histologic examination showed encephalitis to be present in 11 (73%) of the 15 animals studied after 1 week. This model may be used to evaluate the therapeutic efficacy of new antiviral agents and modalities in the treatment of herpes family viral retinitis. The model is most similar to herpes simplex or zoster retinitis in humans, but also shares some similarities (and differences) with cytomegalovirus (CMV) retinitis in humans.
为研究针对疱疹病毒所致视网膜炎的局部玻璃体内治疗方法，需要一种局灶性、亚急性、相对非致死性的疱疹病毒家族性视网膜炎动物模型。将1型单纯疱疹病毒（HSV-1）注入33只荷兰有色兔眼的视网膜下间隙。接种后对动物观察长达42天。所有接种的眼睛均以可预测的方式出现局灶性、不断扩大的视网膜炎区域，表现为视网膜混浊和坏死的局灶性扩大区域，并伴有不同程度的视网膜出血。在接种的眼睛中，所有动物在21天内均发生视网膜脱离；33%的动物未接种的眼睛出现局灶性视网膜炎。组织学检查显示，在研究的15只动物中，11只（73%）在1周后出现脑炎。该模型可用于评估新型抗病毒药物和治疗方法在治疗疱疹病毒家族性病毒性视网膜炎中的疗效。该模型与人类单纯疱疹或带状疱疹性视网膜炎最为相似，但也与人类巨细胞病毒（CMV）视网膜炎有一些相似之处（和不同之处）。
