伪狂犬病病毒糖蛋白II在抵御致死性感染中的作用。
Role of pseudorabies virus glycoprotein II in protection from lethal infection.
作者信息
Nakamura T, Ihara T, Nunoya T, Kuwahara H, Ishihama A, Ueda S
机构信息
Nippon Institute for Biological Science, Tokyo, Japan.
出版信息
Vet Microbiol. 1993 Jul;36(1-2):83-90. doi: 10.1016/0378-1135(93)90130-y.
A monoclonal antibody (mAb), named 1.21, with complement-dependent neutralizing activity was produced against glycoprotein II (gII) of pseudorabies virus (PRV). By immunoaffinity chromatography using a mAB 1.21 column, gII was purified from Nonidet P40-lysates of PRV infected BHK21/13 cells. When mice and pigs were immunized with purified gII, complement-dependent virus-neutralizing antibodies were produced. The immunized animals survived potentially lethal challenge with PRV. These results indicate that an immunological response against gII plays an important role in the protection from PRV infection.
制备了一种名为1.21的单克隆抗体（mAb），它对伪狂犬病病毒（PRV）的糖蛋白II（gII）具有补体依赖性中和活性。通过使用mAb 1.21柱进行免疫亲和层析，从感染PRV的BHK21/13细胞的Nonidet P40裂解物中纯化出gII。用纯化的gII免疫小鼠和猪后，产生了补体依赖性病毒中和抗体。免疫后的动物在受到PRV的潜在致死性攻击后存活下来。这些结果表明，针对gII的免疫反应在预防PRV感染中起着重要作用。
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