Sasajima Y, Yamabe H, Kobashi Y, Hirai K, Mori S
Department of Pathology, University of Tokyo, Japan.
Am J Pathol. 1993 Nov;143(5):1280-5.
Pyothorax-associated lymphoma (PAL) is a rare tumor associated with long-standing tuberculous pyothorax. Most of these lymphomas are B-cell lymphomas of high-grade malignancy. Over 50 cases have been reported in Japan, but no cases have been described in Western countries. Its pathogenesis remains unknown. We studied immunohistologically the expression of Epstein-Barr virus- (EBV) encoded latent gene products, EB nuclear antigen-2 and LMP-1, in four cases of PAL. Fifty B-cell lymphomas unrelated to pyothorax, and five EBV-bearing lymphoblastic tumors produced in severe combined immune deficient mice (severe combined immune deficient-EBV+ tumors) were also studied as controls. Marked expression of EB nuclear antigen-2 was demonstrated on all four PALs. LMP-1 was also present in all cases, but both the staining intensity and the number of stained cells remained less than on severe combined immune deficient-EBV+ tumors. Neither EB nuclear antigen-2 nor LMP-1 was observed in the 50 control B-cell lymphomas. Additional molecular genetic analysis revealed that EBVs are incorporated into each PAL clonally. These results confirm the definite association of EBV with PALs, although the significance of weak expression of LMP-1 awaits further study.
脓胸相关淋巴瘤（PAL）是一种与长期结核性脓胸相关的罕见肿瘤。这些淋巴瘤大多是高度恶性的B细胞淋巴瘤。日本已报道50多例，但西方国家尚无相关病例描述。其发病机制尚不清楚。我们对4例PAL进行了免疫组织化学研究，检测爱泼斯坦-巴尔病毒（EBV）编码的潜伏基因产物EB核抗原-2和LMP-1的表达。还研究了50例与脓胸无关的B细胞淋巴瘤以及在严重联合免疫缺陷小鼠中产生的5例携带EBV的淋巴母细胞瘤（严重联合免疫缺陷-EBV+肿瘤）作为对照。在所有4例PAL中均显示出EB核抗原-2的明显表达。所有病例中也都存在LMP-1，但染色强度和染色细胞数量均低于严重联合免疫缺陷-EBV+肿瘤。在50例对照B细胞淋巴瘤中未观察到EB核抗原-2和LMP-1。进一步的分子遗传学分析表明，EBV克隆性地整合到每个PAL中。这些结果证实了EBV与PAL之间存在明确关联，尽管LMP-1弱表达的意义有待进一步研究。
