Esteve-Comas M, Núñez M C, Fernández-Bañares F, Abad-Lacruz A, Gil A, Cabré E, González-Huix F, Bertrán X, Gassull M A
Department of Gastroenterology, Hospital Universitari Germans Trias i Pujol, Badalona, Spain.
Gut. 1993 Oct;34(10):1370-3. doi: 10.1136/gut.34.10.1370.
An abnormal plasma polyunsaturated fatty acid pattern (PUFA) (increased n3 and decreased n6 PUFA) has been reported in active inflammatory bowel disease (IBD). The possibility of a primary defect in the PUFA metabolism in IBD was hypothesised. The aim of this study was to assess plasma PUFA pattern in inactive inflammatory bowel disease and to ascertain whether patients who had had a colectomy and who were suffering from ulcerative colitis have a similar PUFA pattern than those patients with non-active ulcerative colitis and who had not had a colectomy. Plasma fatty acids were analysed by semi-capillary column gas-liquid chromatography in three groups of patients with inactive IBD (24 patients with inactive ulcerative colitis who had not had a colectomy, 15 patients with ulcerative colitis who had had a colectomy, and 27 patients with Crohn's disease). Plasma concentration and percentage of C22:6n3 and unsaturation index were significantly higher in patients with inactive ulcerative colitis without a colectomy and the Crohn's disease group (p < 0.0001) than in controls. Plasma concentration and percentage of C22:6n3 and the unsaturation index remained significantly higher, in both the operated and non-operated ulcerative colitis patients when compared with controls (p < 0.0001). These results suggest that in inactive IBD, an increased PUFA biosynthesis might be the cause of the high values of n3 compounds. These findings although seen in active disease, are more noticeable in remission because of the lack of artefactual factors (malnutrition, steroids, inflammation). In addition, persistence of high values in both groups of ulcerative colitis patients--that is, those who had had a colectomy and those who had not suggests the existence of a primary abnormality in the PUFA metabolism in IBD.
据报道，活动性炎症性肠病（IBD）患者存在异常的血浆多不饱和脂肪酸模式（PUFA）（n3增加而n6 PUFA减少）。有人推测IBD中PUFA代谢存在原发性缺陷。本研究的目的是评估非活动性炎症性肠病患者的血浆PUFA模式，并确定接受结肠切除术且患有溃疡性结肠炎的患者与未接受结肠切除术的非活动性溃疡性结肠炎患者的PUFA模式是否相似。通过半毛细管柱气液色谱法分析了三组非活动性IBD患者的血浆脂肪酸（24例未接受结肠切除术的非活动性溃疡性结肠炎患者、15例接受结肠切除术的溃疡性结肠炎患者和27例克罗恩病患者）。未接受结肠切除术的非活动性溃疡性结肠炎患者组和克罗恩病组的血浆C22:6n3浓度、百分比及不饱和度指数显著高于对照组（p<0.0001）。与对照组相比，接受手术和未接受手术的溃疡性结肠炎患者的血浆C22:6n3浓度、百分比及不饱和度指数仍显著更高（p<0.0001）。这些结果表明，在非活动性IBD中，PUFA生物合成增加可能是n3化合物值升高的原因。这些发现虽然在活动性疾病中可见，但由于缺乏人为因素（营养不良、类固醇、炎症），在缓解期更为明显。此外，两组溃疡性结肠炎患者（即接受结肠切除术和未接受结肠切除术的患者）的值持续较高，这表明IBD中PUFA代谢存在原发性异常。
