Li H, Wang D, Mu J
Hua Xi Yi Ke Da Xue Xue Bao. 1993 Jun;24(2):167-70.
According to Jerne's idiotype-anti-idiotype network theory, a new method to detect Anti-HBs was studied. This method utilized monoclonal Anti-Id as a substitute for HBsAg of the routine method adopting the enzyme-linked immunosorbent assay (ELISA) format. The serum specimen was incubated with solid-phase (polyvinyl chloride) that had been coated with monoclonal Anti-Id. If Anti-HBs was present in the serum, it would be combined with the Anti-Id. HRP-labeled goat Anti-human IgG was added, which reacted with the immobilized Anti-HBs. Under optimal conditions, 0.5 microgram/ml Anti-HBs could be detected. This method is safe, simple, and specific for Anti-HBs with no cross-reactivity with Anti-HBs, Anti-HBe and rheumatic factor positive serum, and the reagents can be obtained in unlimited amounts and are homogeneous. These features are particularly attractive when the antigen is difficult to obtain.
根据杰内的独特型-抗独特型网络理论，研究了一种检测抗-HBs的新方法。该方法利用单克隆抗独特型作为常规方法中HBsAg的替代物，采用酶联免疫吸附测定（ELISA）形式。血清标本与已包被单克隆抗独特型的固相（聚氯乙烯）孵育。如果血清中存在抗-HBs，它将与抗独特型结合。加入辣根过氧化物酶（HRP）标记的山羊抗人IgG，其与固定化的抗-HBs反应。在最佳条件下，可检测到0.5微克/毫升的抗-HBs。该方法安全、简单，对抗-HBs具有特异性，与抗-HBs、抗-HBe和风湿因子阳性血清无交叉反应，试剂可大量获得且性质均一。当抗原难以获得时，这些特性尤其具有吸引力。
