Svensson L O, Domeika M, Mårdh P A
Institute of Obstetrics & Gynecology, University Hospital, Uppsala, Sweden.
Genitourin Med. 1993 Oct;69(5):397-9. doi: 10.1136/sti.69.5.397.
To compare a new sampling device, a brush, Accellone-Multi-Instrument (AMI), with a dacron-tipped swab for detection of Chlamydia trachomatis in endocervical specimens, and to evaluate if consecutive multiple cervical sampling as compared with such a single specimen would increase the sensitivity.
501 females attending an STD clinic and 172 females attending a family planning clinic were examined prospectively. Two cervical specimens were collected from each woman. C trachomatis were detected by culture or enzyme immunoassay (IDEIA-III). Positive EIA samples were confirmed by a direct immunofluorescent test.
When cervical specimens were collected with the brush as the first device, 92% of the culture-positive cases were detected, and when the samples were collected with the dacron-tipped-swab first, 84% of the culture-positive cases were detected (p < 0.05). The first collected specimen detected 89% of the culture-positive cases and 81% of those that were positive by IDEIA.
The study indicates that the AMI brush is superior to non-toxic, dacron-tipped swabs for detection of C trachomatis in cervical samples by cell culture but not by ELISA, and that the sensitivity could be improved by analysing multiple cervical samples.
比较一种新型采样装置——Accellone多功能采样器（AMI）毛刷与涤纶头拭子在检测宫颈标本中沙眼衣原体的效果，并评估与单次标本相比，连续多次宫颈采样是否会提高检测敏感性。
对501名到性病门诊就诊的女性和172名到计划生育门诊就诊的女性进行前瞻性检查。每位女性采集两份宫颈标本。采用培养法或酶免疫测定法（IDEIA-III）检测沙眼衣原体。酶免疫测定法阳性的样本通过直接免疫荧光试验进行确认。
当先用毛刷采集宫颈标本时，92%的培养阳性病例被检测出来；而当先使用涤纶头拭子采集样本时，84%的培养阳性病例被检测出来（p<0.05）。首次采集的标本检测出89%的培养阳性病例和81%酶免疫测定法阳性的病例。
该研究表明，在通过细胞培养检测宫颈样本中的沙眼衣原体时，AMI毛刷优于无毒的涤纶头拭子，但在酶联免疫吸附测定中并非如此，并且通过分析多个宫颈样本可提高检测敏感性。
