Kessel D, Morgan A
Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201.
Photochem Photobiol. 1993 Oct;58(4):521-6. doi: 10.1111/j.1751-1097.1993.tb04925.x.
The photophysical and photobiological properties of a series of etiobenzochlorins were evaluated in cell culture using murine leukemia L1210 cells. In the series of agents tested, the chlorin-(mono)sulfonate was the most efficacious, the tin chlorin somewhat less so and the tin chlorin-sulfonate much less active. The parent chlorin was essentially inactive at the limit of solubility. Photodamage was assessed by measuring alterations in surface hydrophobicity (via a two-phase partitioning procedure), amino acid transport and membrane potential. Additional information was provided from fluorescence microscopy, which was used to identify sites of sensitizer binding and effects of photodamage on the binding patterns of fluorescent probes specific for mitochondria, lysosomes and plasma membranes. Effects of photodamage on fluorescence lifetime distribution of the membrane probe trimethylaminodiphenyl hexatriene were examined. The data obtained were consistent with localization of the parnet etiobenzochlorin and tin derivative at lysosomal loci, the chlorin-sulfonate at plasma and mitochondrial membranes and tin-sulfonate at the cell surface.
使用小鼠白血病L1210细胞在细胞培养中评估了一系列埃替苯并二氢卟酚的光物理和光生物学特性。在测试的一系列试剂中，二氢卟酚 - （单）磺酸盐最有效，锡二氢卟酚的效果稍差，而锡二氢卟酚 - 磺酸盐的活性则低得多。母体二氢卟酚在溶解度极限时基本无活性。通过测量表面疏水性的变化（通过两相分配程序）、氨基酸转运和膜电位来评估光损伤。荧光显微镜提供了额外信息，用于识别敏化剂结合位点以及光损伤对线粒体、溶酶体和质膜特异性荧光探针结合模式的影响。研究了光损伤对膜探针三甲基氨基二苯基己三烯荧光寿命分布的影响。获得的数据与母体埃替苯并二氢卟酚和锡衍生物定位于溶酶体位点、二氢卟酚 - 磺酸盐定位于质膜和线粒体膜以及锡 - 磺酸盐定位于细胞表面一致。
