Pedersen A, Madsen H O, Vestergaard B F, Ryder L P
Department of Oral Medicine, University Hospital of Copenhagen (Rigshospitalet), Denmark.
Scand J Dent Res. 1993 Oct;101(5):311-3. doi: 10.1111/j.1600-0722.1993.tb01126.x.
The present study was undertaken to examine for varicella-zoster virus (VZV) constituents in recurrent aphthous ulcers (RAU). Smears and biopsies of ulcers 0-3 days old were obtained from 20 otherwise healthy minor/major RAU patients and from two patients with Behçet's syndrome. VZV could not be cultivated from any of the biopsies, all smears were negative for VZV antigen, and in situ hybridization on the five samples tested was negative for VZV nucleic acid. DNA extracted from biopsies, however, yielded positive polymerase chain reaction (PCR) products in all cases with one primer set of the protein kinase 66 (PK66). Ten control biopsies from non-RAU subjects yielded no PCR products apart from the sample from AP, who obtained the biopsies, and from HOM, who performed the PCR, suggesting that the DNA had been passed on from the patients. Other parts of the PK66 gene and of thymidine kinase 36 yielded no products. Although the consistent presence of VZV DNA in RAU might suggest a pathogenetic implication, the potential presence and possible pathogenetic importance of other herpesviruses cannot be excluded.
本研究旨在检测复发性阿弗他溃疡（RAU）中的水痘-带状疱疹病毒（VZV）成分。从20例其他方面健康的轻度/重度RAU患者以及2例白塞病患者身上获取0 - 3日龄溃疡的涂片和活检样本。所有活检样本均未培养出VZV，所有涂片的VZV抗原检测均为阴性，且所检测的5个样本的原位杂交VZV核酸检测也为阴性。然而，从活检样本中提取的DNA，在使用蛋白激酶66（PK66）的一组引物进行检测时，所有病例均产生了阳性聚合酶链反应（PCR）产物。除了获取活检样本的AP和进行PCR检测的HOM的样本外，来自非RAU受试者的10个对照活检样本均未产生PCR产物，这表明DNA是由患者传递而来的。PK66基因的其他部分以及胸苷激酶36均未产生产物。尽管RAU中持续存在VZV DNA可能提示其具有致病意义，但不能排除其他疱疹病毒的潜在存在及其可能的致病重要性。
