Takaichi M, Nanbo T
Tokai Research Laboratories, Daiichi Pure Chemicals Co. Ltd., Ibaraki, Japan.
Jikken Dobutsu. 1993 Oct;42(4):619-21. doi: 10.1538/expanim1978.42.4_619.
A method of long-term bile collection from unrestrained dogs was developed. A steel needle was passed subcutaneously from the animal's right side into the abdomen. A catheter, threaded through this needle, was inserted from the side and brought out below the abdomen. The catheter was introduced into the common bile duct and a vest was then fitted on the dog. The external catheter tip was placed in a collection bottle in the pocket of the vest. This method allows the animal to move freely in its cage. The bile of an unrestrained dog weighing 10 kg was collected for 3 weeks at an output rate of 4.2-4.6ml per hour.
开发了一种从不受约束的狗身上长期收集胆汁的方法。将一根钢针从动物右侧皮下刺入腹部。将一根导管穿过这根针，从侧面插入并引出到腹部下方。将导管插入胆总管，然后给狗穿上一件背心。外部导管尖端置于背心胸口中的一个收集瓶内。这种方法可使动物在笼中自由活动。对一只体重10千克的不受约束的狗的胆汁进行了为期3周的收集，收集速率为每小时4.2 - 4.6毫升。
