Lorite P, Chica E, Palomeque T
Departamento de Biología Experimental y Ciencias de la Salud, Universidad de Jaén, Spain.
Chromosome Res. 1996 Jan;4(1):77-9. doi: 10.1007/BF02254949.
Well-defined G-bands were obtained on metaphase chromosomes from Tapinoma nigerrimum using trypsin and warm 2 x SSC in sequence. The G-banded pattern allowed the identification of all chromosomes. Evidence for asynchronous condensation of the chromosomes of this species is provided. Different banding patterns were obtained when metaphase chromosomes were stained with DA/DAPI alone and with DA/DAPI after a standard G-banding procedure. The G-banding phenomenon is discussed using the result obtained.
使用胰蛋白酶和温热的2×SSC依次处理黑褐蚁的中期染色体，获得了清晰的G带。G带模式有助于识别所有染色体。提供了该物种染色体异步凝聚的证据。当单独用DA/DAPI以及在标准G带处理后用DA/DAPI对中期染色体进行染色时，获得了不同的带型模式。利用所得结果对G带现象进行了讨论。
