Kilvington S
Public Health Laboratory, Royal United Hospital, UK.
Eye (Lond). 1993;7 ( Pt 4):535-8. doi: 10.1038/eye.1993.116.
Trophozoite and cyst adherence of two Acanthamoeba keratitis strains (PHL/530 and PHL/978) to four types of unworn soft contact lens and their removal by cleaning agents were studied. Greater adherence of the trophozoites compared with the cysts was recorded for both strains. Trophozoites of PHL/530 adhered in greater numbers to type I lenses (61.4% poly[2-hydroxyethyl methacrylate-38.6% water), with no differences between type II (30% lidofilcon A-70% water), III (55% bufilcon A-45% water) and IV lenses (42% etafilcon A-58% water). Adherence of PHL/978 trophozoites to type II lenses was decreased compared with their adherence to the other lenses. Cysts of both strains showed greater adherence to type I and III lenses. Interstrain differences in trophozoite adherence occurred, with PHL/530 showing greater adherence to type I and II lenses. Recommended cleaning procedures using three commercial solutions were effective in removing Acanthamoeba from the lenses. This study indicates the possible role of adherence to contact lenses in the acquisition of Acanthamoeba keratitis, but shows that correct use of commercial cleaning agents may be important in the prevention of infection.
研究了两株棘阿米巴角膜炎菌株（PHL/530和PHL/978）的滋养体和包囊对四种未佩戴过的软性隐形眼镜的黏附情况以及清洁剂对其的清除效果。两株菌株的滋养体黏附情况均比包囊更为明显。PHL/530的滋养体对I型镜片（聚甲基丙烯酸羟乙酯61.4% - 水38.6%）的黏附数量更多，对II型镜片（lidofilcon A 30% - 水70%）、III型镜片（bufilcon A 55% - 水45%）和IV型镜片（etafilcon A 42% - 水58%）的黏附情况无差异。与其他镜片相比，PHL/978滋养体对II型镜片的黏附有所减少。两株菌株的包囊对I型和III型镜片的黏附性更强。滋养体黏附存在菌株间差异，PHL/530对I型和II型镜片的黏附性更强。使用三种商业溶液的推荐清洁程序能有效清除镜片上的棘阿米巴。本研究表明黏附于隐形眼镜可能在棘阿米巴角膜炎的感染中起作用，但正确使用商业清洁剂对预防感染可能很重要。
